Additive effects of CuSO4 and aromatic compounds on laccase production by Pleurotus sajor-caju PS-2001 using sucrose as a carbon source
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ADDITIVE EFFECTS OF CuSO4 AND AROMATIC COMPOUNDS ON LACCASE PRODUCTION BY Pleurotus sajor-caju PS-2001 USING SUCROSE AS A CARBON SOURCE
Laccase enzymes are now commercially available, and a laccase/mediator combination is currently marketed for indigo dye bleaching in textile manufacturing; replacing traditional chemical-based processes with enzymatic technology reduces the need for effluent treatment. However, an inexpensive source of these enzymes will be needed to enable wider application of this technology. In the present w...
متن کاملDifferential regulation of laccase gene expression in Pleurotus sajor-caju.
Four laccase isozyme genes, Psc lac1, 2, 3 and 4 have been cloned from the edible mushroom, Pleurotus sajor-caju. The genes display a high degree of homology with other basidiomycete laccases (55-99%) at the amino acid level. Of the laccase genes isolated, Psc lac1 and 4 displayed the highest degree of similarity (85% at the amino acid level), while Psc lac3 showed the highest degree of diverge...
متن کاملRemazol Brilliant Blue R Dye Decolourization by Laccase Produced by Pleurotus Sajor-caju via Solid-State Fermentation
The application of synthetic dyes in various industries improved the aesthetic properties of the products, but the discharge of dye-containing effluent into water bodies leads to serious environmental problems. Till date, colour removal from effluent is still a challenge due to the absence of economically attractive treatments. In this study, decolourization of a synthetic dye – Remazol brillia...
متن کاملMolecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host.
The Psc lac4 gene from Pleurotus sajor-caju has been cloned and expressed in the heterologous host Pichia pastoris, under the control of the AOX1 methanol inducible promoter. The native Ple. sajor-caju laccase signal sequence was effective in directing the secretion of lac4 expressed in Pic. pastoris. The control of media pH and temperature was found to be important in obtaining sufficient quan...
متن کاملUpgrading of Animal Feed by Solid State Fermentation by Pleurotus sajor-caju
Solid state fermentaton using cellulolytic fungus, Pleurotus sajor-caju with pretreatment of irradiation and lime for upgradation of four agro-wastes such as pulse husk, rice bran, wheat bran and sugarcane bagasse to animal feed were investigated. The fermentation of the substrates was done at 30°C. Total bacteria and fungi were reduced effectively by 10 kGy of radiation dose. Pretreatment with...
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ژورنال
عنوان ژورنال: Brazilian Journal of Chemical Engineering
سال: 2014
ISSN: 0104-6632
DOI: 10.1590/0104-6632.20140312s00002241